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PMID: 10449771 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An inverse relationship between T cell receptor affinity and antigen dose during CD4(+) T cell responses in vivo and in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 17 ·1999-08-17 ·Pages 9781-6

Rees W, Bender J, Teague TK, Kedl RM, Crawford F, Marrack P, Kappler J

Abstract

Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.

MeSH Terms
Animals CD4-Positive T-Lymphocytes/immunology Flow Cytometry Histocompatibility Antigens Class II/immunology Hyaluronan Receptors/immunology Hybridomas/immunology Immunization, Secondary Mice Mice, Inbred C57BL Receptors, Antigen, T-Cell/physiology
Chemicals
Histocompatibility Antigens Class II Hyaluronan Receptors Receptors, Antigen, T-Cell
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rees W
Howard Hughes Medical Institute, Division of Basic Immunology, National Jewish Medical and Research Center, Denver, CO 80206, USA.
Bender J
Teague T K
Kedl R M
Crawford F
Marrack P
Kappler J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.17.9781
Published
1999-08-17
Pages
9781-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22287
Subset
IM
Grants
NIAID NIH HHS · R01 AI018785 · United States
NIAID NIH HHS · AI-17134 · United States
NIAID NIH HHS · AI-18785 · United States
NIAID NIH HHS · R01 AI017134 · United States
NIAID NIH HHS · R37 AI018785 · United States
NIAID NIH HHS · R56 AI017134 · United States
NIAID NIH HHS · AI-22295 · United States
NIAID NIH HHS · R56 AI018785 · United States
NIAID NIH HHS · P01 AI022295 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.17.9781 PMC Full Text View journal details
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