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PMID: 10449366 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Interfacial binding of secreted phospholipases A(2): more than electrostatics and a major role for tryptophan.

Current opinion in structural biology ·Vol. 9 ·No. 4 ·1999-08-00 ·Pages 428-32

Gelb MH, Cho W, Wilton DC

Abstract

Secreted phospholipases A(2) have similar catalytic sites, but vastly different interfacial binding surfaces that modulate their binding affinity for different kinds of phospholipid vesicles by several orders of magnitude. The structure/function principles that dictate both the differential interfacial binding and the physiological function of these enzymes are beginning to be unraveled.

MeSH Terms

Animals Bee Venoms/enzymology Binding Sites Calcium/physiology Catalysis Cattle Cell Membrane/metabolism Crystallography, X-Ray Eicosanoids/metabolism Elapid Venoms/enzymology Humans Liposomes/metabolism Membrane Lipids/metabolism Models, Molecular Mutagenesis, Site-Directed Phospholipases A/chemistry,genetics,metabolism Phospholipids/metabolism Protein Binding Protein Conformation Static Electricity Swine Tryptophan/physiology

Chemicals

Bee Venoms Eicosanoids Elapid Venoms Liposomes Membrane Lipids Phospholipids Tryptophan Phospholipases A Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gelb M H

Departments of Chemistry and Biochemistry, University of Washington, 351700, Seattle, WA 98195, USA. gelb@chem.washington.edu

Cho W

Wilton D C

Article Info

Journal

Current opinion in structural biology

Abbr.

Curr Opin Struct Biol

ISSN

0959-440X

DOI

10.1016/S0959-440X(99)80059-1

gelb@chem.washington.edu

Published

1999-08-00

Pages

428-32

Language

English

Region

England

NLM ID

9107784

Subset

Grants

NIGMS NIH HHS · GM52598 · United States

NIGMS NIH HHS · GM53987 · United States

NHLBI NIH HHS · HL36235 · United States

Wellcome Trust · United Kingdom

Corrections

CommentIn

PMID: 10449379

External Links

PubMed source DOI: 10.1016/S0959-440X(99)80059-1 View journal details

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Interfacial binding of secreted phospholipases A(2): more than electrostatics and a major role for tryptophan.

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