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PMID: 10444594 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cell-counting factor regulating structure size in Dictyostelium.

Genes & development ·Vol. 13 ·No. 15 ·1999-08-01 ·Pages 1960-9

Brock DA, Gomer RH

Abstract

Developing Dictyostelium cells form large aggregation streams that break up into groups of 0.2 x 10(5) to 1 x 10(5) cells. Each group then becomes a fruiting body. smlA cells oversecrete an unknown factor that causes aggregation streams to break up into groups of approximately 5 x 10(3) cells and thus form very small fruiting bodies. We have purified the counting factor and find that it behaves as a complex of polypeptides with an effective molecular mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have named counting. In transformants with a disrupted counting gene, there is no detectable secretion of counting factor, and the aggregation streams do not break up, resulting in huge (up to 2 x 10(5) cell) fruiting bodies.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Aggregation Cell Count Cloning, Molecular Culture Media, Conditioned Dictyostelium/cytology,genetics,growth & development Molecular Sequence Data Molecular Weight Mutation Phenotype Protein Sorting Signals/genetics Protozoan Proteins/chemistry,genetics,isolation & purification,metabolism RNA, Messenger/genetics,metabolism Sequence Analysis Signal Transduction
Chemicals
Culture Media, Conditioned Protein Sorting Signals Protozoan Proteins RNA, Messenger SmlA protein, Dictyostelium discoideum countin protein, Dictyostelium discoideum
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brock D A
Howard Hughes Medical Institute, Department of Biochemistry and Cell Biology MS-140, Rice University, Houston, Texas 77005-1892, USA.
Gomer R H
References (40)
40 references, click to expand
  1. Integrative mechanisms in development of the early chick blastoderm. III. Role of cell population size and growth potentiality in synthetic systems larger than normal.
    J Exp Zool. 1961 Aug;147:271-93 PMID: 14039462
  2. Regulation of extracellular cyclic-AMP-phosphodiesterase activity during development of Dictyostelium discoideum.
    Biochem Biophys Res Commun. 1971 Jan 8;42(1):119-24 PMID: 4322968
  3. A secreted factor and cyclic AMP jointly regulate cell-type-specific gene expression in Dictyostelium discoideum.
    Mol Cell Biol. 1985 Apr;5(4):705-13 PMID: 2985966
  4. The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.
    EMBO J. 1997 Jul 16;16(14):4317-32 PMID: 9250676
  5. A theoretical study of the effects of cyclic AMP phosphodiesterases during aggregation in Dictyostelium.
    J Cell Sci. 1976 Oct;22(1):49-58 PMID: 185228
  6. A secreted 80 x 10(3) Mr protein mediates sensing of cell density and the onset of development in Dictyostelium.
    Development. 1991 May;112(1):269-78 PMID: 1663029
  7. Biochemical and genetic evidence for two extracellular adenosine 3':5'-monophosphate phosphodiesterases in Dictyostelium purpureum.
    Eur J Biochem. 1979 Apr 2;95(2):407-17 PMID: 222583
  8. Cell density-sensing in Dictyostelium by means of the accumulation rate, diffusion coefficient and activity threshold of a protein secreted by starved cells.
    J Theor Biol. 1994 Apr 7;167(3):273-82 PMID: 8207951
  9. The phosphodiesterase secreted by prestalk cells is necessary for Dictyostelium morphogenesis.
    Dev Biol. 1995 Jan;167(1):1-8 PMID: 7851634
  10. Mutagenesis and gene identification in Dictyostelium by shotgun antisense.
    Proc Natl Acad Sci U S A. 1996 May 14;93(10):5003-7 PMID: 8643519
  11. A developmentally regulated cell surface receptor for a density-sensing factor in Dictyostelium.
    J Biol Chem. 1994 Mar 25;269(12):9128-36 PMID: 8132650
  12. Cell aggregation and differentiation in Dictyostelium.
    Curr Top Dev Biol. 1968;3:157-97 PMID: 5002049
  13. A strategy to study development and pattern formation: use of antibodies against products of cloned genes.
    Methods Cell Biol. 1987;28:471-87 PMID: 3298996
  14. Multiple forms of an extracellular cyclic-AMP phosphodiesterase from Dictyostelium discoideum.
    Biochim Biophys Acta. 1979 May 23;578(1):232-42 PMID: 222338
  15. EVIDENCE FOR A SUBSTANCE RESPONSIBLE FOR THE SPACING PATTERN OF AGGREGATION AND FRUITING IN THE CELLULAR SLIME MOLDS.
    J Embryol Exp Morphol. 1963 Sep;11:571-89 PMID: 14061960
  16. A proteinous factor mediating intercellular communication during the transition of Dictyostelium cells from growth to differentiation.
    Zoolog Sci. 1995 Feb;12(1):61-9 PMID: 7795492
  17. Binding of inhibitor alters kinetic and physical properties of extracellular cyclic AMP phosphodiesterase from Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5450-4 PMID: 230476
  18. Regulation and processing of a secreted protein that mediates sensing of cell density in Dictyostelium.
    Development. 1991 Dec;113(4):1375-85 PMID: 1811950
  19. RtoA links initial cell type choice to the cell cycle in Dictyostelium.
    Development. 1996 Nov;122(11):3677-85 PMID: 8951083
  20. Rescue of a Dictyostelium discoideum mutant defective in cyclic nucleotide phosphodiesterase.
    Dev Biol. 1989 Feb;131(2):366-72 PMID: 2536338
  21. PSF and CMF, autocrine factors that regulate gene expression during growth and early development of Dictyostelium.
    Experientia. 1995 Dec 18;51(12):1124-34 PMID: 8536800
  22. A density-sensing factor regulates signal transduction in Dictyostelium.
    J Cell Biol. 1995 Jun;129(5):1251-62 PMID: 7775572
  23. The extracellular cyclic nucleotide phosphodiesterase of Dictyostelium discoideum. Purification and characterization.
    J Biol Chem. 1981 Jul 25;256(14):7620-7 PMID: 6265455
  24. Streamer F mutants and chemotaxis of Dictyostelium.
    Bioessays. 1992 Jul;14(7):473-9 PMID: 1332700
  25. A Dictystelium mutant with defective aggregate size determination.
    Development. 1996 Sep;122(9):2569-78 PMID: 8787732
  26. Overexpression of the csA cell adhesion molecule under its own cAMP-regulated promoter impairs morphogenesis in Dictyostelium.
    J Cell Sci. 1992 Jun;102 ( Pt 2):203-14 PMID: 1328260
  27. The role of the cyclic nucleotide phosphodiesterase of Dictyostelium discoideum during growth, aggregation, and morphogenesis: overexpression and localization studies with the separate promoters of the pde.
    Dev Biol. 1993 May;157(1):73-84 PMID: 8387036
  28. Aggregation in Dictyostelium discoideum.
    Cell. 1981 Jun;24(3):603-11 PMID: 6265098
  29. Defective cyclic adenosine-3', 5'-phosphate-phosphodiesterase regulation in morphogenetic mutants of Dictyostelium discoideum.
    J Mol Biol. 1973 Mar 15;74(4):573-85 PMID: 4354075
  30. Dictyostelium discoideum: a model system for cell-cell interactions in development.
    Science. 1989 Sep 8;245(4922):1054-8 PMID: 2672337
  31. Disruption of Dictyostelium discoideum morphogenesis by overproduction of cAMP phosphodiesterase.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8076-80 PMID: 2847151
  32. Integration of signaling information in controlling cell-fate decisions in Dictyostelium.
    Genes Dev. 1995 Jun 15;9(12):1427-44 PMID: 7601348
  33. Lateral inhibition and pattern formation in Dictyostelium.
    Curr Top Dev Biol. 1993;28:1-46 PMID: 8348838
  34. A density-sensing factor controls development in Dictyostelium.
    Genes Dev. 1992 Mar;6(3):390-400 PMID: 1547939
  35. The cyclic nucleotide phosphodiesterase inhibitory protein of Dictyostelium discoideum. Purification and characterization.
    J Biol Chem. 1981 Jul 25;256(14):7628-37 PMID: 6265456
  36. Cell number control and timing in animal development: the oligodendrocyte cell lineage.
    Int J Dev Biol. 1998;42(3):263-7 PMID: 9654007
  37. A transformation vector for dictyostelium discoideum with a new selectable marker bsr.
    Plasmid. 1993 Sep;30(2):150-4 PMID: 8234487
  38. Cellular differentiation in dictyosteliaceae; heritable modifications of the developmental pattern.
    Ann N Y Acad Sci. 1953 Oct 14;56(5):949-60 PMID: 13139286
  39. Phenotypic changes induced by a mutated ras gene during the development of Dictyostelium transformants.
    Nature. 1986 Sep 25-Oct 1;323(6086):340-3 PMID: 3093890
  40. Developmental signal transduction pathways uncovered by genetic suppressors.
    Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15260-5 PMID: 8986798
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.15.1960
Published
1999-08-01
Pages
1960-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316923
Subset
IM
Databases
GENBANK
AF140780
External Links
PubMed source DOI: 10.1101/gad.13.15.1960 PMC Full Text View journal details
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