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PMID: 10435614 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mdm2 binds p73 alpha without targeting degradation.

Oncogene ·Vol. 18 ·No. 27 ·1999-07-08 ·Pages 3923-9

Bálint E, Bates S, Vousden KH

Abstract

The function of the p53 tumor suppressor protein is regulated by interaction with Mdm2, which targets p53 for ubiquitin dependent degradation. We show here that like p53, p73 alpha forms an interaction with Mdm2, both in vitro and in cells, but this does not result in the degradation of the p73 alpha protein. The human papillomavirus E6 protein also fails to degrade p73 alpha, suggesting that the mechanisms governing p73 alpha stability are distinct from those known to regulate p53 stability. However, the interaction of Mdm2 with 73 alpha is sufficient to impede p73 alpha transcriptional function, despite the lack of degradation.

MeSH Terms

Amino Acid Sequence Cysteine Endopeptidases/metabolism Cysteine Proteinase Inhibitors/pharmacology DNA-Binding Proteins/antagonists & inhibitors,genetics,metabolism Genes, Tumor Suppressor Humans Molecular Sequence Data Multienzyme Complexes/metabolism Nuclear Proteins/antagonists & inhibitors,genetics,metabolism Oncogene Proteins, Viral/physiology Osteosarcoma Papillomaviridae Proteasome Endopeptidase Complex Protein Isoforms/genetics,metabolism Proto-Oncogene Proteins/genetics,metabolism,physiology Proto-Oncogene Proteins c-mdm2 Repressor Proteins/physiology Transcription, Genetic Transfection Tumor Cells, Cultured Tumor Protein p73 Tumor Suppressor Proteins

Chemicals

Cysteine Proteinase Inhibitors DNA-Binding Proteins E6 protein, Human papillomavirus type 16 Multienzyme Complexes Nuclear Proteins Oncogene Proteins, Viral Protein Isoforms Proto-Oncogene Proteins Repressor Proteins TP73 protein, human Tumor Protein p73 Tumor Suppressor Proteins MDM2 protein, human Proto-Oncogene Proteins c-mdm2 Cysteine Endopeptidases Proteasome Endopeptidase Complex

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bálint E

ABL Basic Research Program, NCI-FCRDC, Frederick, Maryland, 21702-1202, USA.

Bates S

Vousden K H

Article Info

Journal

Oncogene

Abbr.

Oncogene

ISSN

0950-9232

DOI

10.1038/sj.onc.1202781

Published

1999-07-08

Pages

3923-9

Language

English

Region

England

NLM ID

8711562

Subset

External Links

PubMed source DOI: 10.1038/sj.onc.1202781 View journal details

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