Cultured rat microglia express functional beta-chemokine receptors.

Journal of neuroimmunology ·Vol. 98 ·No. 2 ·1999-08-03 ·Pages 176-84

Boddeke EW, Meigel I, Frentzel S, Gourmala NG, Harrison JK, Buttini M, Spleiss O, Gebicke-Härter P

Abstract

We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia.

MeSH Terms

Animals Calcium/metabolism Calcium Signaling/immunology Cells, Cultured Chemokine CCL2/pharmacology Chemokine CCL3 Chemokine CCL4 Chemokine CCL5/pharmacology Cytosol/metabolism DNA Primers Enzyme Inhibitors/pharmacology Gene Expression/drug effects,immunology Lipopolysaccharides/pharmacology Macrophage Inflammatory Proteins/pharmacology Microglia/chemistry,cytology,immunology RNA, Messenger/analysis Rats Rats, Sprague-Dawley Receptors, CCR1 Receptors, CCR2 Receptors, CCR5/genetics,immunology Receptors, Chemokine/genetics,immunology Recombinant Proteins/pharmacology Reverse Transcriptase Polymerase Chain Reaction Ryanodine/pharmacology Thapsigargin/pharmacology Virulence Factors, Bordetella/pharmacology

Chemicals

Ccr1 protein, rat Ccr2 protein, rat Chemokine CCL2 Chemokine CCL3 Chemokine CCL4 Chemokine CCL5 DNA Primers Enzyme Inhibitors Lipopolysaccharides Macrophage Inflammatory Proteins RNA, Messenger Receptors, CCR1 Receptors, CCR2 Receptors, CCR5 Receptors, Chemokine Recombinant Proteins Virulence Factors, Bordetella Ryanodine Thapsigargin Calcium

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Boddeke E W

Department of Medical Physiology, University of Groningen, The Netherlands. h.w.g.m.boddeke@med.rug.nl

Meigel I

Frentzel S

Gourmala N G

Harrison J K

Buttini M

Spleiss O

Gebicke-Härter P

Article Info

Journal

Journal of neuroimmunology

Abbr.

J Neuroimmunol

ISSN

0165-5728

DOI

10.1016/s0165-5728(99)00096-x

h.w.g.m.boddeke@med.rug.nl

Published

1999-08-03

Pages

176-84

Language

English

Region

Netherlands

NLM ID

8109498

Subset

External Links

PubMed source DOI: 10.1016/s0165-5728(99)00096-x View journal details

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Cultured rat microglia express functional beta-chemokine receptors.

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