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PMID: 10427741 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dilute spin-exchange assignment of solid-state NMR spectra of oriented proteins: acetylcholine M2 in bilayers.

Journal of biomolecular NMR ·Vol. 14 ·No. 2 ·1999-06-00 ·Pages 141-8

Marassi FM, Gesell JJ, Valente AP, Kim Y, Oblatt-Montal M, Montal M, Opella SJ

Abstract

The assignment of amide resonances in the two-dimensional PISEMA (Polarization Inversion with Spin Exchange at the Magic Angle) spectrum of uniformly 15N labeled M2 peptide corresponding to the channel-lining segment of the acetylcholine receptor in oriented phospholipid bilayers is described. The majority of the resonances were assigned through comparisons with spectra from selectively 15N labeled recombinant peptides and specifically 15N labeled synthetic peptides. Some resonances were assigned to specific amino acid residues by means of homonuclear 15N spin-exchange spectroscopy. A modification to the conventional spin-exchange pulse sequence that significantly shortens the length of the experiments by combining the intervals for 15N spin-exchange and 1H magnetization recovery is described.

MeSH Terms
Amino Acid Sequence Lipid Bilayers Molecular Sequence Data Nitrogen Isotopes Nuclear Magnetic Resonance, Biomolecular/instrumentation,methods Peptide Fragments/chemistry Protein Conformation Receptor, Muscarinic M2 Receptors, Muscarinic/chemistry
Chemicals
Lipid Bilayers Nitrogen Isotopes Peptide Fragments Receptor, Muscarinic M2 Receptors, Muscarinic
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Marassi F M
Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA.
Gesell J J
Valente A P
Kim Y
Oblatt-Montal M
Montal M
Opella S J
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1023/a:1008391823293
Published
1999-06-00
Pages
141-8
Language
English
Region
Netherlands
NLM ID
9110829
PMCID
PMC3282054
Subset
IM
Grants
NIGMS NIH HHS · P01 GM056538-03 · United States
NCRR NIH HHS · P41 RR009793-06 · United States
NIGMS NIH HHS · R01 GM049711 · United States
NIBIB NIH HHS · P41 EB002031 · United States
NIGMS NIH HHS · R01GM-29754 · United States
NIGMS NIH HHS · P01GM-56538 · United States
NIGMS NIH HHS · R01 GM029754 · United States
NIGMS NIH HHS · R01 GM029754-18 · United States
NIGMS NIH HHS · R01GM-49711 · United States
External Links
PubMed source DOI: 10.1023/a:1008391823293 PMC Full Text View journal details
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