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PMID: 10427076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR.

Applied and environmental microbiology ·Vol. 65 ·No. 8 ·1999-08-00 ·Pages 3735-7

Romero C, Lopez-Goñi I

Abstract

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.

MeSH Terms
Animals Bacteriological Techniques Brucella/genetics,isolation & purification Cattle Colony Count, Microbial DNA, Bacterial/genetics,isolation & purification Female Milk/microbiology Polymerase Chain Reaction/methods
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Romero C
Departamento de Microbiología, Universidad de Navarra, 31008 Pamplona, Spain.
Lopez-Goñi I
References (8)
8 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.65.8.3735-3737.1999
Published
1999-08-00
Pages
3735-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC91561
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.65.8.3735-3737.1999 PMC Full Text View journal details
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