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PMID: 10427070 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis.

Applied and environmental microbiology ·Vol. 65 ·No. 8 ·1999-08-00 ·Pages 3710-3

Hausner M, Wuertz S

Abstract

Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.

MeSH Terms
Bacteria/genetics Biofilms Conjugation, Genetic Gene Transfer Techniques Genes, Reporter Green Fluorescent Proteins Luminescent Proteins/genetics Microscopy, Confocal
Chemicals
Luminescent Proteins Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hausner M
Institute of Water Quality Control and Waste Management, Technical University of Munich, D-85748 Garching, Germany.
Wuertz S
References (27)
27 references, click to expand
  1. Effect of bacterial distribution and activity on conjugal gene transfer on the phylloplane of the bush bean (Phaseolus vulgaris).
    Appl Environ Microbiol. 1998 May;64(5):1902-9 PMID: 9572970
  2. The acquisition of indigenous plasmids by a genetically marked pseudomonad population colonizing the sugar beet phytosphere is related to local environmental conditions.
    Appl Environ Microbiol. 1997 Apr;63(4):1577-83 PMID: 16535580
  3. Green fluorescent protein as a marker for gene expression.
    Science. 1994 Feb 11;263(5148):802-5 PMID: 8303295
  4. In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes.
    Appl Environ Microbiol. 1993 Jul;59(7):2293-8 PMID: 8357261
  5. The involvement of cell-to-cell signals in the development of a bacterial biofilm.
    Science. 1998 Apr 10;280(5361):295-8 PMID: 9535661
  6. The kinetics of conjugative plasmid transmission: fit of a simple mass action model.
    Plasmid. 1979 Apr;2(2):247-60 PMID: 377323
  7. Transfer in Marine Sediments of the Naturally Occurring Plasmid pRAS1 Encoding Multiple Antibiotic Resistance.
    Appl Environ Microbiol. 1994 Dec;60(12):4234-8 PMID: 16349453
  8. The green fluorescent protein.
    Annu Rev Biochem. 1998;67:509-44 PMID: 9759496
  9. Plasmid transfer between strains of Pseudomonas aeruginosa on membrane filters attached to river stones.
    J Gen Microbiol. 1987 Nov;133(11):3099-107 PMID: 3128637
  10. Survival strategies of bacteria in the natural environment.
    Microbiol Rev. 1987 Sep;51(3):365-79 PMID: 3312987
  11. Effect of growth phase and parental cell survival in river water on plasmid transfer between Escherichia coli strains.
    Appl Environ Microbiol. 1994 Dec;60(12):4273-8 PMID: 7811066
  12. Establishment of new genetic traits in a microbial biofilm community.
    Appl Environ Microbiol. 1998 Jun;64(6):2247-55 PMID: 9603843
  13. Intergeneric transfer of conjugative and mobilizable plasmids harbored by Escherichia coli in the gut of the soil microarthropod Folsomia candida (Collembola).
    Appl Environ Microbiol. 1998 Jul;64(7):2652-9 PMID: 9647844
  14. Alcaligenes eutrophus as a bacterial chromate sensor.
    Appl Environ Microbiol. 1998 Feb;64(2):453-8 PMID: 9464379
  15. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
    Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
  16. Automated confocal laser scanning microscopy and semiautomated image processing for analysis of biofilms.
    Appl Environ Microbiol. 1998 Nov;64(11):4115-27 PMID: 9797255
  17. Improved green fluorescence.
    Nature. 1995 Feb 23;373(6516):663-4 PMID: 7854443
  18. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  19. Low-frequency horizontal transfer of an element containing the chlorocatechol degradation genes from Pseudomonas sp. strain B13 to Pseudomonas putida F1 and to indigenous bacteria in laboratory-scale activated-sludge microcosms.
    Appl Environ Microbiol. 1998 Jun;64(6):2126-32 PMID: 9603824
  20. Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
    J Bacteriol. 1985 Apr;162(1):328-34 PMID: 3884593
  21. In Situ Detection of High Levels of Horizontal Plasmid Transfer in Marine Bacterial Communities.
    Appl Environ Microbiol. 1998 Jul 1;64(7):2670-5 PMID: 9647846
  22. Construction and characterization of heavy metal-resistant haloaromatic-degrading Alcaligenes eutrophus strains.
    Appl Environ Microbiol. 1993 Jan;59(1):334-9 PMID: 8439161
  23. Bacterial plasmid conjugation on semi-solid surfaces monitored with the green fluorescent protein (GFP) from Aequorea victoria as a marker.
    Gene. 1996;173(1 Spec No):59-65 PMID: 8707058
  24. Temperature, pH, and cations affect the ability of Escherichia coli to mobilize plasmids in L broth and synthetic wastewater.
    Can J Microbiol. 1987 Aug;33(8):733-7 PMID: 3319117
  25. Microbial biofilms.
    Annu Rev Microbiol. 1995;49:711-45 PMID: 8561477
  26. Understanding, improving and using green fluorescent proteins.
    Trends Biochem Sci. 1995 Nov;20(11):448-55 PMID: 8578587
  27. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov.
    Microbiol Immunol. 1995;39(11):897-904 PMID: 8657018
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.65.8.3710-3713.1999
Published
1999-08-00
Pages
3710-3
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC91555
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.65.8.3710-3713.1999 PMC Full Text View journal details
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