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PMID: 10388813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conservation of ARS elements and chromosomal DNA replication origins on chromosomes III of Saccharomyces cerevisiae and S. carlsbergensis.

Genetics ·Vol. 152 ·No. 3 ·1999-07-00 ·Pages 933-41

Yang C, Theis JF, Newlon CS

Abstract

DNA replication origins, specified by ARS elements in Saccharomyces cerevisiae, play an essential role in the stable transmission of chromosomes. Little is known about the evolution of ARS elements. We have isolated and characterized ARS elements from a chromosome III recovered from an alloploid Carlsberg brewing yeast that has diverged from its S. cerevisiae homeologue. The positions of seven ARS elements identified in this S. carlsbergensis chromosome are conserved: they are located in intergenic regions flanked by open reading frames homologous to those that flank seven ARS elements of the S. cerevisiae chromosome. The S. carlsbergensis ARS elements were active both in S. cerevisiae and S. monacensis, which has been proposed to be the source of the diverged genome present in brewing yeast. Moreover, their function as chromosomal replication origins correlated strongly with the activity of S. cerevisiae ARS elements, demonstrating the conservation of ARS activity and replication origin function in these two species.

MeSH Terms
Conserved Sequence DNA Replication/physiology DNA-Binding Proteins/genetics Electrophoresis, Gel, Two-Dimensional Models, Genetic Replication Origin Saccharomyces/genetics Saccharomyces cerevisiae/genetics Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yang C
Department of Microbiology and Molecular Genetics, New Jersey Medical School and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA.
Theis J F
Newlon C S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/152.3.933
Published
1999-07-00
Pages
933-41
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460674
Subset
IM
Grants
NIGMS NIH HHS · GM-35679 · United States
NHGRI NIH HHS · HG-00027 · United States
External Links
PubMed source DOI: 10.1093/genetics/152.3.933 PMC Full Text View journal details
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