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PMID: 10385524 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule.

The Journal of cell biology ·Vol. 145 ·No. 7 ·1999-06-28 ·Pages 1443-59

Luetterforst R, Stang E, Zorzi N, Carozzi A, Way M, Parton RG

Abstract

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH2 terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32-amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.

MeSH Terms
Amino Acid Sequence Animals Antibodies Caveolin 1 Caveolin 3 Caveolins Cell Line Cell Membrane/chemistry,drug effects,metabolism,ultrastructure Cells, Cultured Conserved Sequence/genetics Fibroblasts Fluorescent Antibody Technique Golgi Apparatus/chemistry,drug effects,metabolism,ultrastructure Humans Intracellular Membranes/chemistry,drug effects,metabolism,ultrastructure Membrane Proteins/analysis,chemistry,genetics,metabolism Microscopy, Immunoelectron Molecular Sequence Data Nocodazole/pharmacology Palmitic Acid/metabolism Protein Binding Protein Sorting Signals Recombinant Fusion Proteins/analysis,biosynthesis,immunology Sequence Deletion Transfection
Chemicals
Antibodies CAV1 protein, human Caveolin 1 Caveolin 3 Caveolins Membrane Proteins Protein Sorting Signals Recombinant Fusion Proteins Palmitic Acid Nocodazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Luetterforst R
Centre for Microscopy and Microanalysis, Department of Physiology and Pharmacology, and Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Queensland 4072, Australia.
Stang E
Zorzi N
Carozzi A
Way M
Parton R G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.145.7.1443
Published
1999-06-28
Pages
1443-59
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133166
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.145.7.1443 PMC Full Text View journal details
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