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PMID: 10364365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy.

Journal of virology ·Vol. 73 ·No. 7 ·1999-07-00 ·Pages 6099-103

Lewin SR, Vesanen M, Kostrikis L, Hurley A, Duran M, Zhang L, Ho DD, Markowitz M

Abstract

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

MeSH Terms
Adult Anti-HIV Agents/therapeutic use CD4 Lymphocyte Count HIV Infections/drug therapy,immunology,virology HIV-1/genetics,physiology Humans Leukocytes, Mononuclear/virology Longitudinal Studies RNA, Messenger/analysis RNA, Viral/analysis Reverse Transcriptase Polymerase Chain Reaction/methods Time Factors Virus Replication
Chemicals
Anti-HIV Agents RNA, Messenger RNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lewin S R
Aaron Diamond AIDS Research Center, New York, New York 10016, USA.
Vesanen M
Kostrikis L
Hurley A
Duran M
Zhang L
Ho D D
Markowitz M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.7.6099-6103.1999
Published
1999-07-00
Pages
6099-103
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112674
Subset
IM
Grants
NIAID NIH HHS · AI41534 · United States
NCRR NIH HHS · M01 RR000102 · United States
NCRR NIH HHS · MO1-RR00102 · United States
NIAID NIH HHS · U01 AI041534 · United States
NIAID NIH HHS · P30 AI042848 · United States
NIAID NIH HHS · IP60AI42848-01 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.7.6099-6103.1999 PMC Full Text View journal details
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