Biodiversity in deep-sea sites located near the south part of Japan.

Extremophiles : life under extreme conditions ·Vol. 3 ·No. 2 ·1999-05-00 ·Pages 97-102

Takami H, Kobata K, Nagahama T, Kobayashi H, Inoue A, Horikoshi K

Abstract

We obtained 100 isolates of bacteria from deep-sea mud samples collected at various depths (1050-10897m). Various types of bacteria such as alkaliphiles, thermophiles, psychrophiles, and halophiles were recovered on agar plates at a frequency of 0.8 x 10(2) to 2.3 x 10(4)/ g of dry sea mud. No acidophiles were recovered. These extremophilic bacteria were widely distributed, being detected at each deep-sea site, and the frequency of isolation of such extremophiles from the deep-sea mud was not directly influenced by the depth of the sampling sites. Phylogenetic analysis of deep-sea isolates based on 16S rDNA sequences revealed that a wide range of taxa were represented in the deep-sea environments. Growth patterns under high hydrostatic pressure were determined for the deep-sea isolates obtained in this study. No extremophilic strains isolated in this study showed growth at 60MPa, although a few of the other isolates grew slightly at this hydrostatic pressure.

MeSH Terms

Bacteria/classification,genetics,growth & development,isolation & purification Base Sequence DNA, Bacterial DNA, Ribosomal/analysis Japan Molecular Sequence Data Pacific Ocean Pressure RNA, Ribosomal, 16S Sequence Analysis, DNA Water Microbiology

Chemicals

DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Takami H

Deep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, Yokosuka. takamih@jamstec.go.jp

Kobata K

Nagahama T

Kobayashi H

Inoue A

Horikoshi K

Article Info

Journal

Extremophiles : life under extreme conditions

Abbr.

Extremophiles

ISSN

1431-0651

DOI

10.1007/s007920050104

takamih@jamstec.go.jp

Published

1999-05-00

Pages

97-102

Language

English

Region

Germany

NLM ID

9706854

Subset

Databases

GENBANK

AB010842, AB010843, AB010845, AB010846, AB010847, AB010848, AB010850, AB010851, AB010852, AB010853, AB010854, AB010858, AB010859, AB010860, AB010861, AB010862, AB010863, AB010864, AB010865, AB010866, AB010867, AB010868, AB010869, AB010870

External Links

PubMed source DOI: 10.1007/s007920050104 View journal details

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Biodiversity in deep-sea sites located near the south part of Japan.

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