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PMID: 10356395 Published · ppublish English Journal Article Comment

Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins.

Science (New York, N.Y.) ·Vol. 284 ·No. 5420 ·1999-06-04 ·Pages 1664-6

Tortorella MD, Burn TC, Pratta MA, Abbaszade I, Hollis JM, Liu R, Rosenfeld SA, Copeland RA, Decicco CP, Wynn R, Rockwell A, Yang F, Duke JL, Solomon K, George H, Bruckner R, Nagase H, Itoh Y, Ellis DM, Ross H, Wiswall BH, Murphy K, Hillman MC, Hollis GF, Newton RC, Magolda RL, Trzaskos JM, Arner EC

Abstract

We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373-alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.

MeSH Terms

ADAM Proteins ADAMTS1 Protein ADAMTS4 Protein Aggrecans Amino Acid Sequence Arthritis/drug therapy Cartilage/metabolism Catalytic Domain Cloning, Molecular Disintegrins/chemistry,metabolism Extracellular Matrix Proteins Humans Hydroxamic Acids/pharmacology Interleukin-1/pharmacology Lectins, C-Type Metalloendopeptidases/chemistry,genetics,isolation & purification,metabolism Molecular Sequence Data Procollagen N-Endopeptidase Protease Inhibitors/pharmacology Protein Sorting Signals Proteoglycans/metabolism Recombinant Proteins/chemistry,metabolism Sequence Analysis

Chemicals

Aggrecans Disintegrins Extracellular Matrix Proteins Hydroxamic Acids Interleukin-1 Lectins, C-Type Protease Inhibitors Protein Sorting Signals Proteoglycans Recombinant Proteins ADAM Proteins ADAMTS1 Protein ADAMTS1 protein, human Metalloendopeptidases Procollagen N-Endopeptidase ADAMTS4 Protein ADAMTS4 protein, human

Authors & Affiliations

28 authors, click to expand affiliations / ORCID

Tortorella M D

Department of Inflammatory Diseases Research, DuPont Pharmaceuticals Company, Wilmington, DE 19880-0400, USA.

Burn T C

Pratta M A

Abbaszade I

Hollis J M

Liu R

Rosenfeld S A

Copeland R A

Decicco C P

Wynn R

Rockwell A

Yang F

Duke J L

Solomon K

George H

Bruckner R

Nagase H

Itoh Y

Ellis D M

Ross H

Wiswall B H

Murphy K

Hillman M C

Hollis G F

Newton R C

Magolda R L

Trzaskos J M

Arner E C

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.284.5420.1664

Published

1999-06-04

Pages

1664-6

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

AF148213

Corrections

CommentOn

PMID: 10383332

External Links

PubMed source DOI: 10.1126/science.284.5420.1664 View journal details

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Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins.

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