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PMID: 10346817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Assembly of functionally active Drosophila origin recognition complex from recombinant proteins.

Genes & development ·Vol. 13 ·No. 10 ·1999-05-15 ·Pages 1289-96

Chesnokov I, Gossen M, Remus D, Botchan M

Abstract

In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of origin usage in Drosophila is not regulated solely by a large family of different ORC proteins. The six-subunit ORC can be reconstituted with recombinant proteins into a complex that restores DNA replication in ORC-depleted Drosophila or Xenopus egg extracts.

MeSH Terms
Amino Acid Sequence Animals Chromosome Mapping Cloning, Molecular DNA Replication/physiology DNA-Binding Proteins/analysis,genetics Drosophila/genetics Male Molecular Sequence Data Origin Recognition Complex Ovum/metabolism Recombinant Fusion Proteins/genetics Sequence Homology, Amino Acid Spermatozoa/metabolism Time Factors Xenopus/metabolism
Chemicals
DNA-Binding Proteins Origin Recognition Complex Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chesnokov I
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 USA.
Gossen M
Remus D
Botchan M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.10.1289
Published
1999-05-15
Pages
1289-96
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316721
Subset
IM
Grants
NCI NIH HHS · CA09041 · United States
NIGMS NIH HHS · R01 GM097052 · United States
NCI NIH HHS · R37 CA030490 · United States
NCI NIH HHS · T32 CA009041 · United States
NCI NIH HHS · CA30490 · United States
NCI NIH HHS · R01 CA030490 · United States
Databases
GENBANK
AF139062, AF139063, AF139064
External Links
PubMed source DOI: 10.1101/gad.13.10.1289 PMC Full Text View journal details
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