Perturbation of RET signaling in the embryonic kidney.

Developmental genetics ·Vol. 24 ·No. 3-4 ·1999-00-00 ·Pages 263-72

Ehrenfels CW, Carmillo PJ, Orozco O, Cate RL, Sanicola M

Abstract

We have used a RET-Ig fusion protein to disrupt signaling in the rat embryonic kidney development pathway. Treatment of embryonic kidney organ cultures with RET-Ig results in a decrease in branching of the ureteric bud and a down regulation in expression of the Wnt-11, Wnt-4, and ld genes. These data suggest that Wnt-11, Wnt-4, and ld function downstream of RET signaling in normal development. Expression of BMP-7, shh, and ptc were uneffected by RET-Ig treatment, implying that these genes are regulated independently of ret. We have also performed immunohistochemistry with a GFR alpha-1 specific polyclonal antisera to localize GFR alpha-1 protein expression in the developing kidney.

MeSH Terms

Animals Base Sequence Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins/genetics DNA Primers/genetics Drosophila Proteins Fetal Proteins/genetics Formins Gene Expression Regulation, Developmental Glial Cell Line-Derived Neurotrophic Factor Receptors Glycoproteins/genetics Hedgehog Proteins Humans Immunohistochemistry In Situ Hybridization Kidney/embryology,metabolism Membrane Proteins/genetics Microfilament Proteins Nuclear Proteins/genetics Organ Culture Techniques Patched Receptors Proteins/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-ret Rats Receptor Protein-Tyrosine Kinases/genetics Receptors, Cell Surface Recombinant Fusion Proteins/genetics Signal Transduction Trans-Activators Transforming Growth Factor beta Wnt Proteins Wnt4 Protein

Chemicals

BMP7 protein, human Bmp7 protein, rat Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins DNA Primers Drosophila Proteins Fetal Proteins Formins GFRA1 protein, human Gfra1 protein, rat Glial Cell Line-Derived Neurotrophic Factor Receptors Glycoproteins Hedgehog Proteins Membrane Proteins Microfilament Proteins Nuclear Proteins Patched Receptors Proteins Proto-Oncogene Proteins Receptors, Cell Surface Recombinant Fusion Proteins SHH protein, human Trans-Activators Transforming Growth Factor beta WNT4 protein, human Wnt Proteins Wnt4 Protein Wnt4 protein, rat Proto-Oncogene Proteins c-ret Receptor Protein-Tyrosine Kinases Ret protein, Drosophila Ret protein, rat

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Ehrenfels C W

Department of Molecular Genetics, Biogen, Inc., Cambridge, Massachusetts 02142, USA.

Carmillo P J

Orozco O

Cate R L

Sanicola M

Article Info

Journal

Developmental genetics

Abbr.

Dev Genet

ISSN

0192-253X

DOI

10.1002/(SICI)1520-6408(1999)24:3/4<263::AID-DVG9>3.0.CO;2-D

Published

1999-00-00

Pages

263-72

Language

English

Region

United States

NLM ID

7909963

Subset

External Links

PubMed source DOI: 10.1002/(SICI)1520-6408(1999)24:3/4<263::AID-DVG9>3.0.CO;2-D

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