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PMID: 10318913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 10 ·1999-05-11 ·Pages 5504-9

Shinohara T, Avarbock MR, Brinster RL

Abstract

Although spermatogenesis is essential for reproduction, little is known about spermatogonial stem cells. These cells provide the basis for spermatogenesis throughout adult life by undergoing self-renewal and by providing progeny that differentiate into spermatozoa. A major impediment to our understanding of the biology of these stem cells is the inability to distinguish them from spermatogonia that are committed to differentiation. We made use of the known association of stem cells with basement membranes and our spermatogonial transplantation assay system to identify specific molecular markers on the stem cell surface. Selection of mouse testis cells with anti-beta1- or anti-alpha6-integrin antibody, but not anti-c-kit antibody, produced cell populations with a significantly enhanced ability to colonize recipient testes and generate donor cell-derived spermatogenesis. We demonstrate spermatogonial stem cell-associated antigens by using an assay system based on biological function. Furthermore, the presence of surface integrins on spermatogonial stem cells suggests that these cells share elements of a common molecular machinery with stem cells in other tissues.

MeSH Terms
Animals Antigens, CD/analysis Biomarkers/analysis Cell Differentiation Cell Line Cell Transplantation Extracellular Matrix Proteins/metabolism Flow Cytometry Integrin alpha6 Integrin beta1/analysis Male Mice Mice, Transgenic Proto-Oncogene Proteins c-kit/immunology Spermatogenesis Spermatogonia/immunology Stem Cells/immunology
Chemicals
Antigens, CD Biomarkers Extracellular Matrix Proteins Integrin alpha6 Integrin beta1 Proto-Oncogene Proteins c-kit
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shinohara T
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Avarbock M R
Brinster R L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.10.5504
Published
1999-05-11
Pages
5504-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21889
Subset
IM
Grants
NICHD NIH HHS · HD 36504 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.10.5504 PMC Full Text View journal details
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