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PMID: 1025156 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes.

The Journal of cell biology ·Vol. 68 ·No. 3 ·1976-03-00 ·Pages 781-7

Hoffstein S, Soberman R, Goldstein I, Weissmann G

Abstract

Human neutrophils stimulated by concanavalin A (Con A, 100 microng/ml) contained markedly enhanced numbers of microtubules and discharged peroxidase-negative (specific) but not peroxidase-position (azurophile) granules. Release of lysozyme from specific granules was dose and time dependent, could be inhibitied by alpha-methyl-D-mannoside, and enhanced by cytochalasin B. Many microtubules were associated with internalized plasma membrane bearing Con A binding sites.

MeSH Terms
Cell Survival Concanavalin A/pharmacology Cytochalasin B/pharmacology Dose-Response Relationship, Drug Humans Methylmannosides/pharmacology Microtubules/drug effects,metabolism Muramidase/metabolism Neutrophils/drug effects,enzymology,ultrastructure Temperature
Chemicals
Methylmannosides Concanavalin A Cytochalasin B Muramidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hoffstein S
Soberman R
Goldstein I
Weissmann G
References (18)
18 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.68.3.781
Published
1976-03-00
Pages
781-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109658
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.68.3.781 PMC Full Text View journal details
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