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PMID: 10233979 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of monoclonal antibodies raised against the latent nuclear antigen of human herpesvirus 8.

Journal of virology ·Vol. 73 ·No. 6 ·1999-06-00 ·Pages 5149-55

Kellam P, Bourboulia D, Dupin N, Shotton C, Fisher C, Talbot S, Boshoff C, Weiss RA

Abstract

Human herpesvirus 8 (HHV-8; also designated Kaposi's sarcoma-associated herpesvirus) is the likely etiological agent of Kaposi's sarcoma (KS). HHV-8 encodes a latent nuclear antigen (LNA) which is the product of the viral gene orf 73. LNA is recognized by most infected patient sera and is the basis of current immunofluorescence assays used in epidemiological studies of HHV-8 infection. Here we describe the characterization of four monoclonal antibodies raised to the C-terminal third of LNA-glutathione S-transferase fusion proteins. These monoclonal antibodies recognized discrete linear epitopes within the C terminus and repetitive region of LNA, detected antigen in primary effusion lymphoma (PEL) cells, and precipitated a 220- to 230-kDa protein doublet corresponding to LNA from HHV-8-infected PEL cell lines. In situ immunocytochemistry of KS lesions with these antibodies show that LNA is extensively expressed in KS spindle cells.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Viral/analysis,physiology Cell Line Epitope Mapping Fluorescent Antibody Technique Herpesvirus 8, Human/immunology Humans Precipitin Tests Sarcoma, Kaposi/virology
Chemicals
Antibodies, Monoclonal Antigens, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kellam P
Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom. p.Kellam@ucl.ac.uk
Bourboulia D
Dupin N
Shotton C
Fisher C
Talbot S
Boshoff C
Weiss R A
References (31)
31 references, click to expand
  1. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi's sarcoma.
    Nat Med. 1996 Aug;2(8):925-8 PMID: 8705864
  2. Seroconversion to antibodies against Kaposi's sarcoma-associated herpesvirus-related latent nuclear antigens before the development of Kaposi's sarcoma.
    N Engl J Med. 1996 Jul 25;335(4):233-41 PMID: 8657239
  3. Establishment and characterization of a primary effusion (body cavity-based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus.
    Blood. 1996 Oct 1;88(7):2648-54 PMID: 8839859
  4. Prevalence of Kaposi's sarcoma associated herpesvirus infection measured by antibodies to recombinant capsid protein and latent immunofluorescence antigen.
    Lancet. 1996 Oct 26;348(9035):1133-8 PMID: 8888167
  5. Molecular mimicry of human cytokine and cytokine response pathway genes by KSHV.
    Science. 1996 Dec 6;274(5293):1739-44 PMID: 8939871
  6. Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8).
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14862-7 PMID: 8962146
  7. The prevalence of serum antibody to human herpesvirus 8 (Kaposi sarcoma-associated herpesvirus) among HIV-seropositive and high-risk HIV-seronegative women.
    JAMA. 1997 Feb 12;277(6):478-81 PMID: 9020272
  8. Identification of two homologs of the Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in retroperitoneal fibromatosis of different macaque species.
    J Virol. 1997 May;71(5):4138-44 PMID: 9094697
  9. Open reading frame 26 of human herpesvirus 8 encodes a tetradecanoyl phorbol acetate- and butyrate-inducible 32-kilodalton protein expressed in a body cavity-based lymphoma cell line.
    J Virol. 1997 Jun;71(6):4791-7 PMID: 9151873
  10. The 222- to 234-kilodalton latent nuclear protein (LNA) of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) is encoded by orf73 and is a component of the latency-associated nuclear antigen.
    J Virol. 1997 Aug;71(8):5915-21 PMID: 9223481
  11. A herpesvirus of rhesus monkeys related to the human Kaposi's sarcoma-associated herpesvirus.
    J Virol. 1997 Dec;71(12):9764-9 PMID: 9371642
  12. Identification of the gene encoding the major latency-associated nuclear antigen of the Kaposi's sarcoma-associated herpesvirus.
    J Clin Invest. 1997 Nov 15;100(10):2606-10 PMID: 9366576
  13. Transcription mapping of the Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) genome in a body cavity-based lymphoma cell line (BC-1).
    J Virol. 1998 Feb;72(2):1005-12 PMID: 9444993
  14. Identification and characterization of human herpesvirus-8 lytic cycle-associated ORF 59 protein and the encoding cDNA by monoclonal antibody.
    Virology. 1998 Jan 5;240(1):118-26 PMID: 9448696
  15. Establishing a KSHV+ cell line (BCP-1) from peripheral blood and characterizing its growth in Nod/SCID mice.
    Blood. 1998 Mar 1;91(5):1671-9 PMID: 9473233
  16. Kaposi's sarcoma (KS), KS-associated herpesvirus, and the criteria for causality in the age of molecular biology.
    Am J Epidemiol. 1998 Feb 1;147(3):217-21 PMID: 9482495
  17. Human herpesvirus 8 seroprevalence in blood donors and lymphoma patients from different regions of Italy.
    J Natl Cancer Inst. 1998 Mar 4;90(5):395-7 PMID: 9498490
  18. Restricted expression of Epstein-Barr virus (EBV)-encoded, growth transformation-associated antigens in an EBV- and human herpesvirus type 8-carrying body cavity lymphoma line.
    J Gen Virol. 1998 Jun;79 ( Pt 6):1445-52 PMID: 9634087
  19. Kaposi's sarcoma-associated herpesvirus.
    Adv Cancer Res. 1998;75:57-86 PMID: 9709807
  20. A cluster of latently expressed genes in Kaposi's sarcoma-associated herpesvirus.
    J Virol. 1998 Oct;72(10):8309-15 PMID: 9733875
  21. Identification of a major latent nuclear antigen, LNA-1, in the human herpesvirus 8 genome.
    J Hum Virol. 1997 Nov-Dec;1(1):19-29 PMID: 10195227
  22. Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma.
    Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4546-51 PMID: 10200299
  23. Primary structure of the herpesvirus saimiri genome.
    J Virol. 1992 Aug;66(8):5047-58 PMID: 1321287
  24. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma.
    Science. 1994 Dec 16;266(5192):1865-9 PMID: 7997879
  25. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas.
    N Engl J Med. 1995 May 4;332(18):1186-91 PMID: 7700311
  26. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman's disease.
    Blood. 1995 Aug 15;86(4):1276-80 PMID: 7632932
  27. A role for a new herpes virus (KSHV) in different forms of Kaposi's sarcoma.
    Nat Med. 1995 Jul;1(7):707-8 PMID: 7585156
  28. Kaposi's sarcoma-associated herpesvirus infects endothelial and spindle cells.
    Nat Med. 1995 Dec;1(12):1274-8 PMID: 7489408
  29. HHV8 and skin cancers in immunosuppressed patients.
    Lancet. 1996 Feb 3;347(8997):338-9 PMID: 8569406
  30. Antibodies to butyrate-inducible antigens of Kaposi's sarcoma-associated herpesvirus in patients with HIV-1 infection.
    N Engl J Med. 1996 May 16;334(20):1292-7 PMID: 8609946
  31. Antibodies to human herpesvirus type 8 in the general population and in Kaposi's sarcoma patients.
    Lancet. 1996 Sep 28;348(9031):858-61 PMID: 8826812
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.6.5149-5155.1999
Email
p.kellam@ucl.ac.uk
Published
1999-06-00
Pages
5149-55
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112561
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.6.5149-5155.1999 PMC Full Text View journal details
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