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PMID: 10233164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The formation of an insulin-responsive vesicular cargo compartment is an early event in 3T3-L1 adipocyte differentiation.

Molecular biology of the cell ·Vol. 10 ·No. 5 ·1999-05-00 ·Pages 1581-94

El-Jack AK, Kandror KV, Pilch PF

Abstract

Differentiating 3T3-L1 cells exhibit a dramatic increase in the rate of insulin-stimulated glucose transport during their conversion from proliferating fibroblasts to nonproliferating adipocytes. On day 3 of 3T3-L1 cell differentiation, basal glucose transport and cell surface transferrin binding are markedly diminished. This occurs concomitant with the formation of a distinct insulin-responsive vesicular pool of intracellular glucose transporter 1 (GLUT1) and transferrin receptors as assessed by sucrose velocity gradients. The intracellular distribution of the insulin-responsive aminopeptidase is first readily detectable on day 3, and its gradient profile and response to insulin at this time are identical to that of GLUT1. With further time of differentiation, GLUT4 is expressed and targeted to the same insulin-responsive vesicles as the other three proteins. Our data are consistent with the notion that a distinct insulin-sensitive vesicular cargo compartment forms early during fat call differentiation and its formation precedes GLUT4 expression. The development of this compartment may result from the differentiation-dependent inhibition of constitutive GLUT1 and transferrin receptor trafficking such that there is a large increase in, or the new formation of, a population of postendosomal, insulin-responsive vesicles.

MeSH Terms
3T3 Cells/cytology,drug effects,metabolism Adipocytes/cytology,drug effects,metabolism Aminopeptidases/drug effects,metabolism Androstadienes/pharmacology Animals Antibodies/pharmacology Biological Transport Cell Compartmentation/drug effects,physiology Cell Differentiation/physiology Deoxyglucose/metabolism Glucose/metabolism Glucose Transporter Type 1 Glucose Transporter Type 4 Insulin/metabolism,pharmacology Insulin Antagonists/pharmacology Mice Monosaccharide Transport Proteins/immunology,metabolism Muscle Proteins Receptors, Transferrin/metabolism Transferrin/metabolism Wortmannin
Chemicals
Androstadienes Antibodies Glucose Transporter Type 1 Glucose Transporter Type 4 Insulin Insulin Antagonists Monosaccharide Transport Proteins Muscle Proteins Receptors, Transferrin Slc2a1 protein, mouse Slc2a4 protein, mouse Transferrin Deoxyglucose Aminopeptidases Glucose Wortmannin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
El-Jack A K
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Kandror K V
Pilch P F
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.5.1581
Published
1999-05-00
Pages
1581-94
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25345
Subset
IM
Grants
NIDDK NIH HHS · DK-30425 · United States
NIDDK NIH HHS · DK-52057 · United States
NIDDK NIH HHS · R01 DK030425 · United States
NIDDK NIH HHS · R56 DK052057 · United States
NIDDK NIH HHS · R01 DK052057 · United States
External Links
PubMed source DOI: 10.1091/mbc.10.5.1581 PMC Full Text View journal details
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