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PMID: 10224252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interorganelle signaling is a determinant of longevity in Saccharomyces cerevisiae.

Genetics ·Vol. 152 ·No. 1 ·1999-05-00 ·Pages 179-90

Kirchman PA, Kim S, Lai CY, Jazwinski SM

Abstract

Replicative capacity, which is the number of times an individual cell divides, is the measure of longevity in the yeast Saccharomyces cerevisiae. In this study, a process that involves signaling from the mitochondrion to the nucleus, called retrograde regulation, is shown to determine yeast longevity, and its induction resulted in postponed senescence. Activation of retrograde regulation, by genetic and environmental means, correlated with increased replicative capacity in four different S. cerevisiae strains. Deletion of a gene required for the retrograde response, RTG2, eliminated the increased replicative capacity. RAS2, a gene previously shown to influence longevity in yeast, interacts with retrograde regulation in setting yeast longevity. The molecular mechanism of aging elucidated here parallels the results of genetic studies of aging in nematodes and fruit flies, as well as the caloric restriction paradigm in mammals, and it underscores the importance of metabolic regulation in aging, suggesting a general applicability.

MeSH Terms
Antifungal Agents/pharmacology Antimycin A/pharmacology Blotting, Northern Cell Nucleus/metabolism Cell Survival Gene Expression Regulation, Fungal Genes, Reporter Mitochondria/metabolism Organelles/physiology Plasmids Saccharomyces cerevisiae/physiology Signal Transduction Time Factors ras Proteins/metabolism
Chemicals
Antifungal Agents Antimycin A ras Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kirchman P A
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans, Louisiana 70112, USA.
Kim S
Lai C Y
Jazwinski S M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/152.1.179
Published
1999-05-00
Pages
179-90
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460582
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/152.1.179 PMC Full Text View journal details
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