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PMID: 10224037 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis.

Applied and environmental microbiology ·Vol. 65 ·No. 5 ·1999-05-00 ·Pages 2272-5

Hänninen ML, Hakkinen M, Rautelin H

Abstract

The genomic stability of 12 Campylobacter jejuni strains consisting of two groups of human and chicken isolates was studied by analysis of their PFGE (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks' intestines. The patterns of SmaI, SalI, and SacII digests remained stable after intestinal passage, except for those of two strains. One originally human strain, FB 6371, changed its genotype from II/A (SmaI/SacII) to I/B. Another strain, BTI, originally isolated from a chicken, changed its genotype from I/B to a new genotype. The genomic instability of the strains was further confirmed by SalI digestion and ribotyping of the HaeIII digests. In addition, heat-stable serotype 57 of strain FB 6371 changed to serotype 27 in all isolates with new genotypes but remained unchanged in an isolate with the original genotype. Serotype 27 of strain BTI remained stable. Our study suggests that during intestinal colonization, genomic rearrangement, as demonstrated by changed PFGE and ribopatterns, may occur.

MeSH Terms

Animals Campylobacter jejuni/classification,genetics Chickens DNA, Bacterial/genetics,isolation & purification Electrophoresis, Gel, Pulsed-Field Gene Rearrangement Genes, Bacterial Genotype Humans Intestines/microbiology Serotyping

Chemicals

DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hänninen M L

Department of Food and Environmental Hygiene, The Haartman Institute, 00231 Helsinki, Finland. marja-liisa.hanninen@helsinki.fi

Hakkinen M

Rautelin H

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.65.5.2272-2275.1999

marja-liisa.hanninen@helsinki.fi

Published

1999-05-00

Pages

2272-5

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC91334

Subset

External Links

PubMed source DOI: 10.1128/AEM.65.5.2272-2275.1999 PMC Full Text View journal details

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Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis.

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