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PMID: 10217777 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Staphylococcus aureus rsbW (orf159) gene encodes an anti-sigma factor of SigB.

Journal of bacteriology ·Vol. 181 ·No. 9 ·1999-05-00 ·Pages 2846-51

Miyazaki E, Chen JM, Ko C, Bishai WR

Abstract

SigB, a newly discovered alternative sigma factor of Staphylococcus aureus, has been shown to play an important role in stress responses and the regulation of virulence factors. The rsbW (orf159) gene is immediately upstream of sigB. Its gene product is homologous to Bacillus subtilis RsbW which under appropriate conditions binds to B. subtilis SigB and functions as an anti-sigma factor or negative posttranslational regulator. To define the function of S. aureus RsbW, both the S. aureus SigB and RsbW proteins were expressed in Escherichia coli and purified. Cross-linking experiments with these purified proteins revealed that RsbW was capable of specific binding to SigB. In an in vitro transcription runoff assay, RsbW prevented SigB-directed transcription from the sar P3 promoter, a known SigB-dependent promoter, and the inhibitory activity of RsbW was found to be concentration dependent. We also identified SigB promoter consensus sequences upstream of the genes encoding alkaline shock protein 23 and coagulase and have demonstrated SigB and RsbW dependence for the promoters in vitro. These results show that RsbW is a protein sequestering anti-sigma factor of S. aureus SigB and suggest that SigB activity in S. aureus is regulated posttranslationally.

MeSH Terms
Bacterial Proteins/antagonists & inhibitors,biosynthesis,isolation & purification,metabolism Carrier Proteins/genetics,isolation & purification,metabolism Coagulase/biosynthesis Cross-Linking Reagents Gene Expression Regulation, Bacterial Genes, Bacterial Promoter Regions, Genetic Protein Binding Protein Processing, Post-Translational Recombinant Proteins/isolation & purification,metabolism Sigma Factor/antagonists & inhibitors,isolation & purification,metabolism Staphylococcus aureus/genetics Transcription Factors/antagonists & inhibitors,isolation & purification,metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Coagulase Cross-Linking Reagents Recombinant Proteins RsbW protein, bacteria SigB protein, Bacteria Sigma Factor Transcription Factors asp23 protein, Staphylococcus aureus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miyazaki E
Center for Tuberculosis Research, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland 21205-2179, USA.
Chen J M
Ko C
Bishai W R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.9.2846-2851.1999
Published
1999-05-00
Pages
2846-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93728
Subset
IM
Grants
NIAID NIH HHS · R01 AI036973 · United States
NIAID NIH HHS · R01 AI037856 · United States
NIAID NIH HHS · AI36973 · United States
NIAID NIH HHS · AI37856 · United States
External Links
PubMed source DOI: 10.1128/JB.181.9.2846-2851.1999 PMC Full Text View journal details
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