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PMID: 10207161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Roles of TH1 and TH2 cytokines in a murine model of allergic dermatitis.

The Journal of clinical investigation ·Vol. 103 ·No. 8 ·1999-04-00 ·Pages 1103-11

Spergel JM, Mizoguchi E, Oettgen H, Bhan AK, Geha RS

Abstract

Skin lesions in atopic dermatitis (AD) are characterized by hypertrophy of the dermis and epidermis, infiltration by T cells and eosinophils, and expression of the cytokines IL-4, IL-5, and IFN-gamma. The role of these cytokines in the pathogenesis of AD is not known. We took advantage of a recently described murine model of AD elicited by epicutaneous sensitization with ovalbumin (OVA) (1) and of the availability of mice with targeted deletions of the IL-4, IL-5, and IFN-gamma cytokine genes to assess the role of these cytokines in this model.OVA-sensitized skin from IL-5(-/-) mice had no detectable eosinophils and exhibited decreased epidermal and dermal thickening. Sensitized skin from IL-4(-/-) mice displayed normal thickening of the skin layers but had a drastic reduction in eosinophils and a significant increase in infiltrating T cells. These findings were associated with a reduction in eotaxin mRNA and an increase in mRNA for the T-cell chemokines macrophage inflammatory protein-2 (MIP-2), MIP-1beta, and RANTES. Sensitized skin from IFN-gamma-/- mice was characterized by reduced dermal thickening. These results suggest that both the TH2 cytokines IL-4 and IL-5 and the TH1 cytokine IFN-gamma play important roles in the inflammation and hypertrophy of the skin in AD.

MeSH Terms
Allergens/immunology Animals Cricetinae Dermatitis, Atopic/immunology,pathology Disease Models, Animal Eosinophils/immunology Female Interferon-gamma/genetics,immunology Interleukin-4/genetics,immunology Interleukin-5/genetics,immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Ovalbumin/immunology Rats Skin/immunology,pathology Th1 Cells/immunology Th2 Cells/immunology
Chemicals
Allergens Interleukin-5 Interleukin-4 Interferon-gamma Ovalbumin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Spergel J M
Division of Immunology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mizoguchi E
Oettgen H
Bhan A K
Geha R S
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI5669
Published
1999-04-00
Pages
1103-11
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC408277
Subset
IM
Grants
NIAID NIH HHS · U19 AI031541 · United States
NIAID NIH HHS · T32 AI007512 · United States
NIDDK NIH HHS · DK-43551 · United States
NIDDK NIH HHS · DK-47677 · United States
NIAID NIH HHS · P01 AI031541 · United States
NIAID NIH HHS · R01 AI031136 · United States
NICHD NIH HHS · P30HD27805 · United States
NIDDK NIH HHS · R01 DK047677 · United States
External Links
PubMed source DOI: 10.1172/JCI5669 PMC Full Text View journal details
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