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PMID: 10197990 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

NahW, a novel, inducible salicylate hydroxylase involved in mineralization of naphthalene by Pseudomonas stutzeri AN10.

Journal of bacteriology ·Vol. 181 ·No. 8 ·1999-04-00 ·Pages 2315-22

Bosch R, Moore ER, García-Valdés E, Pieper DH

Abstract

Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.

MeSH Terms
Amino Acid Sequence Biodegradation, Environmental Enzyme Induction Genes, Bacterial Isoenzymes/biosynthesis,classification,genetics Mixed Function Oxygenases/biosynthesis,classification,genetics Molecular Sequence Data Naphthalenes/metabolism Pseudomonas/enzymology,genetics Salicylates/metabolism Sequence Homology, Amino Acid Substrate Specificity
Chemicals
Isoenzymes Naphthalenes Salicylates naphthalene Mixed Function Oxygenases salicylate 1-monooxygenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bosch R
Departament de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats, 07071, Palma de Mallorca, Spain.
Moore E R
García-Valdés E
Pieper D H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.8.2315-2322.1999
Published
1999-04-00
Pages
2315-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93652
Subset
IM
Databases
GENBANK
AF039534
External Links
PubMed source DOI: 10.1128/JB.181.8.2315-2322.1999 PMC Full Text View journal details
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