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PMID: 10096082 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A vital stain for studying membrane dynamics in bacteria: a novel mechanism controlling septation during Bacillus subtilis sporulation.

Molecular microbiology ·Vol. 31 ·No. 4 ·1999-02-00 ·Pages 1149-59

Pogliano J, Osborne N, Sharp MD, Abanes-De Mello A, Perez A, Sun YL, Pogliano K

Abstract

At the onset of sporulation in Bacillus subtilis, two potential division sites are assembled at each pole, one of which will be used to synthesize the asymmetrically positioned sporulation septum. Using the vital stain FM 4-64 to label the plasma membrane of living cells, we examined the fate of these potential division sites in wild-type cells and found that, immediately after the formation of the sporulation septum, a partial septum was frequently synthesized within the mother cell at the second potential division site. Using time-lapse deconvolution microscopy, we were able to watch these partial septa first appear and then disappear during sporulation. Septal dissolution was dependent on sigma E activity and was partially inhibited in mutants lacking the sigma E-controlled proteins SpoIID, SpoIIM and SpoIIP, which may play a role in mediating the degradation of septal peptidoglycan. Our results support a model in which sigma E inhibits division at the second potential division site by two distinct mechanisms: inhibition of septal biogenesis and the degradation of partial septa formed before sigma E activation.

MeSH Terms
Bacillus subtilis/genetics,physiology,ultrastructure Bacterial Proteins/genetics,metabolism Cell Cycle Cell Membrane/metabolism Fluorescent Dyes/metabolism Gene Expression Regulation, Bacterial Image Processing, Computer-Assisted Microscopy, Electron Photomicrography Pyridinium Compounds/metabolism Quaternary Ammonium Compounds/metabolism Sigma Factor/metabolism Spores, Bacterial/ultrastructure Time Factors Transcription Factors/metabolism
Chemicals
Bacterial Proteins FM 4-64 Fluorescent Dyes Pyridinium Compounds Quaternary Ammonium Compounds Sigma Factor Transcription Factors spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus sporulation-specific sigma factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pogliano J
Department of Biology, University of California, San Diego 92093-0349, USA.
Osborne N
Sharp M D
Abanes-De Mello A
Perez A
Sun Y L
Pogliano K
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Article Info
Journal
Molecular microbiology
Abbr.
Mol Microbiol
ISSN
0950-382X
DOI
10.1046/j.1365-2958.1999.01255.x
Published
1999-02-00
Pages
1149-59
Language
English
Region
England
NLM ID
8712028
PMCID
PMC2885269
Subset
IM
Grants
NIGMS NIH HHS · R01 GM057045 · United States
NIGMS NIH HHS · R01 GM057045-02 · United States
NIGMS NIH HHS · R01 GM057045-03 · United States
NIGMS NIH HHS · GM-57045 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2958.1999.01255.x PMC Full Text View journal details
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