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PMID: 10095101 Published · ppublish English Journal Article

Ballistic transformation of Caenorhabditis elegans.

Gene ·Vol. 229 ·No. 1-2 ·1999-03-18 ·Pages 31-5

Wilm T, Demel P, Koop HU, Schnabel H, Schnabel R

Abstract

A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.

MeSH Terms
Animals Animals, Genetically Modified Biolistics Caenorhabditis elegans/genetics Caenorhabditis elegans Proteins Collagen/genetics Gold Helium Helminth Proteins/genetics Microscopy, Phase-Contrast Mutation/genetics Particle Size Transformation, Genetic
Chemicals
Caenorhabditis elegans Proteins Helminth Proteins rol-6 protein, C elegans Helium Gold Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wilm T
Max Planck Institut für Biochemie, 82152, Martinsried, Germany.
Demel P
Koop H U
Schnabel H
Schnabel R
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/s0378-1119(99)00043-8
Published
1999-03-18
Pages
31-5
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/s0378-1119(99)00043-8 View journal details
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