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PMID: 10085057 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells.

Infection and immunity ·Vol. 67 ·No. 4 ·1999-04-00 ·Pages 2040-4

Vallis AJ, Finck-Barbançon V, Yahr TL, Frank DW

Abstract

A strain of Pseudomonas aeruginosa that fails to express known type III-secreted effector proteins was constructed as an expression host. Individual effectors were expressed in trans, and their biological effects on CHO cells were assessed in an acute cellular infection model. Intoxication with ExoS, ExoT, or ExoY resulted in alterations in cell morphology. As shown in previous genetic studies, ExoU expression was linked to acute cytotoxicity.

MeSH Terms
Animals Bacterial Toxins/metabolism Biological Transport CHO Cells Cricetinae Exotoxins/metabolism Pseudomonas aeruginosa/metabolism
Chemicals
Bacterial Toxins Exotoxins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vallis A J
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
Finck-Barbançon V
Yahr T L
Frank D W
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22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.4.2040-2044.1999
Published
1999-04-00
Pages
2040-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96567
Subset
IM
Grants
NIAID NIH HHS · AI-01289 · United States
NIAID NIH HHS · AI-31665 · United States
External Links
PubMed source DOI: 10.1128/IAI.67.4.2040-2044.1999 PMC Full Text View journal details
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