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PMID: 10079256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human ligands of the Notch receptor.

The American journal of pathology ·Vol. 154 ·No. 3 ·1999-03-00 ·Pages 785-94

Gray GE, Mann RS, Mitsiadis E, Henrique D, Carcangiu ML, Banks A, Leiman J, Ward D, Ish-Horowitz D, Artavanis-Tsakonas S

Abstract

During development, the Notch signaling pathway is essential for the appropriate differentiation of many cell types in organisms across the phylogenetic scale, including humans. Notch signaling is also implicated in human diseases, including a leukemia and two hereditary syndromes known as Alagille and CADASIL. To generate tools for pursuing the role of the Notch pathway in human disease and development, we have cloned and analyzed the expression of three human homologues of the Notch ligands Delta and Serrate, human Jagged1 (HJ1), human Jagged2 (HJ2), and human Delta1 (H-Delta-1), and determined their chromosomal localizations. We have also raised antibodies to HJ1, and used these antibodies in conjunction with in situ hybridization to examine the expression of these ligands in normal and cancerous cervical tissue. We find that, as reported previously for Notch, the ligands are up-regulated in certain neoplastic tissues. This observation is consistent with the notion that Notch signaling is an important element in these pathogenic conditions, raising the possibility that modulation of Notch activity could be used to influence the fate of the cells and offering a conceivable therapeutic avenue.

MeSH Terms
Amino Acid Sequence/genetics Blotting, Northern Calcium-Binding Proteins Carrier Proteins/genetics,metabolism Cervix Uteri/metabolism Chromosome Mapping Cloning, Molecular Female Humans Intercellular Signaling Peptides and Proteins Intracellular Signaling Peptides and Proteins Jagged-1 Protein Jagged-2 Protein Membrane Proteins/genetics,metabolism Molecular Sequence Data Proteins/genetics,metabolism Reference Values Serrate-Jagged Proteins Uterine Cervical Neoplasms/metabolism
Chemicals
Calcium-Binding Proteins Carrier Proteins Intercellular Signaling Peptides and Proteins Intracellular Signaling Peptides and Proteins JAG1 protein, human JAG2 protein, human Jagged-1 Protein Jagged-2 Protein Membrane Proteins Proteins Serrate-Jagged Proteins delta protein
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Gray G E
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
Mann R S
Mitsiadis E
Henrique D
Carcangiu M L
Banks A
Leiman J
Ward D
Ish-Horowitz D
Artavanis-Tsakonas S
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36 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)65325-4
Published
1999-03-00
Pages
785-94
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1866435
Subset
IM
Grants
NINDS NIH HHS · 2R01NS26804-06A1 · United States
Databases
GENBANK
AF003521, AF003522, U61276
External Links
PubMed source DOI: 10.1016/S0002-9440(10)65325-4 PMC Full Text View journal details
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