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PMID: 10072386 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex.

Genes & development ·Vol. 13 ·No. 5 ·1999-03-01 ·Pages 581-92

Zhang D, Rosbash M

Abstract

In the yeast commitment complex and the mammalian E complex, there is an important base-pairing interaction between the 5' end of U1 snRNA and the conserved 5' splice site region of pre-mRNA. But no protein contacts between splicing proteins and the pre-mRNA substrate have been defined in or near this region of early splicing complexes. To address this issue, we used 4-thiouridine-substituted 5' splice site-containing RNAs as substrates and identified eight cross-linked proteins, all of which were identified previously as commitment complex components. The proteins were localized to three domains: the exon, the six nucleotides of the 5' ss region, and the downstream intron. The results indicate that the 5' splice site region and environs are dense with protein contacts in the commitment complex and suggest that some of them make important contributions to formation or stability of the U1 snRNP-pre-mRNA complex.

MeSH Terms
Base Sequence Chromosome Mapping Fungal Proteins/metabolism Molecular Sequence Data RNA Precursors RNA, Fungal/metabolism Ribonucleoprotein, U1 Small Nuclear/metabolism
Chemicals
Fungal Proteins RNA Precursors RNA, Fungal Ribonucleoprotein, U1 Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang D
Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA.
Rosbash M
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39 references, click to expand
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.5.581
Published
1999-03-01
Pages
581-92
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316503
Subset
IM
Grants
NIGMS NIH HHS · R01 GM023549 · United States
NIGMS NIH HHS · GM23549 · United States
External Links
PubMed source DOI: 10.1101/gad.13.5.581 PMC Full Text View journal details
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