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PMID: 10049902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In situ analysis of phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno (Switzerland).

Applied and environmental microbiology ·Vol. 65 ·No. 3 ·1999-03-00 ·Pages 1325-30

Tonolla M, Demarta A, Peduzzi R, Hahn D

Abstract

Comparative sequence analysis of a 16S rRNA gene clone library from the chemocline of the meromictic Lake Cadagno (Switzerland) revealed the presence of a diverse number of phototrophic sulfur bacteria. Sequences resembled those of rRNA of type strains Chromatium okenii DSM169 and Amoebobacter purpureus DSM4197, as well as those of four bacteria forming a tight cluster with A. purpureus DSM4197 and Lamprocystis roseopersicina DSM229. In situ hybridization with fluorescent (Cy3 labeled) oligonucleotide probes indicated that all large-celled phototrophic sulfur bacteria in the chemocline of Lake Cadagno were represented by C. okenii DSM169, while small-celled phototrophic sulfur bacteria consisted of four major populations with different distribution profiles in the chemocline indicating different ecophysiological adaptations.

MeSH Terms
Bacteria/classification,genetics DNA, Bacterial/analysis,genetics DNA, Ribosomal/analysis Fresh Water Genes, rRNA In Situ Hybridization, Fluorescence Molecular Sequence Data Oligonucleotide Probes Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Switzerland Water Microbiology
Chemicals
DNA, Bacterial DNA, Ribosomal Oligonucleotide Probes RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tonolla M
Cantonal Institute of Bacteriology, Microbial Ecology (University of Geneva), CH-6904 Lugano, Switzerland. mauro.tonolla@ti.ch
Demarta A
Peduzzi R
Hahn D
References (15)
15 references, click to expand
  1. The neighbor-joining method: a new method for reconstructing phylogenetic trees.
    Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
  2. Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments.
    Appl Environ Microbiol. 1996 Apr;62(4):1405-15 PMID: 8919802
  3. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  4. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization.
    Appl Environ Microbiol. 1996 Jun;62(6):2138-44 PMID: 16535341
  5. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
    Appl Environ Microbiol. 1997 Sep;63(9):3367-73 PMID: 9292986
  6. Distribution of bacterial populations in a stratified fjord (Mariager Fjord, Denmark) quantified by in situ hybridization and related to chemical gradients in the water column.
    Appl Environ Microbiol. 1996 Apr;62(4):1391-404 PMID: 8919801
  7. Factors determining annual changes in bacterial photosynthetic pigments in holomictic lake cisó, Spain.
    Appl Environ Microbiol. 1983 Nov;46(5):999-1006 PMID: 16346436
  8. Use of nuclepore filters for counting bacteria by fluorescence microscopy.
    Appl Environ Microbiol. 1977 May;33(5):1225-8 PMID: 327932
  9. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
    J Bacteriol. 1990 Feb;172(2):762-70 PMID: 1688842
  10. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  11. Characterization of the dominant and rare members of a young Hawaiian soil bacterial community with small-subunit ribosomal DNA amplified from DNA fractionated on the basis of its guanine and cytosine composition.
    Appl Environ Microbiol. 1998 Apr;64(4):1283-9 PMID: 9546163
  12. Dual staining of natural bacterioplankton with 4',6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences.
    Appl Environ Microbiol. 1992 Jul;58(7):2158-63 PMID: 1379029
  13. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
    Appl Environ Microbiol. 1990 Jun;56(6):1919-25 PMID: 2200342
  14. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  15. Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow).
    Appl Environ Microbiol. 1996 Jun;62(6):1998-2005 PMID: 8787398
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.65.3.1325-1330.1999
Email
mauro.tonolla@ti.ch
Published
1999-03-00
Pages
1325-30
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC91183
Subset
IM
Databases
GENBANK
AJ006057, AJ006058, AJ006059, AJ006060, AJ006061, AJ006062, AJ006063, AJ006221, AJ223234, AJ223235
External Links
PubMed source DOI: 10.1128/AEM.65.3.1325-1330.1999 PMC Full Text View journal details
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