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PMID: 10049857 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of two plasmids in the degradation of carbaryl by Arthrobacter sp. strain RC100.

Applied and environmental microbiology ·Vol. 65 ·No. 3 ·1999-03-00 ·Pages 1015-9

Hayatsu M, Hirano M, Nagata T

Abstract

A bacterium capable of utilizing carbaryl (1-naphthyl N-methylcarbamate) as the sole carbon source was isolated from carbaryl-treated soil. This bacterium was characterized taxonomically as Arthrobacter and was designated strain RC100. RC100 hydrolyzes the N-methylcarbamate linkage to 1-naphthol, which was further metabolized via salicylate and gentisate. Strain RC100 harbored three plasmids (designated pRC1, pRC2, and pRC3). Mutants unable to degrade carbaryl arose at a high frequency after treating the culture with mitomycin C. All carbaryl-hydrolysis-deficient mutants (Cah-) lacked pRC1, and all 1-naphthol-utilization-deficient mutants (Nat-) lacked pRC2. The plasmid-free strain RC107 grew on gentisate as a carbon source. These two plasmids could be transferred to Cah- mutants or Nat- mutants by conjugation, resulting in the restoration of the Cah and Nah phenotypes.

MeSH Terms
Arthrobacter/genetics,isolation & purification,metabolism Biodegradation, Environmental Carbamates/metabolism Carbaryl/metabolism Conjugation, Genetic DNA, Bacterial/analysis,genetics DNA, Ribosomal/analysis,genetics Insecticides/metabolism Molecular Sequence Data Oxygen Consumption Plasmids/genetics RNA, Ribosomal, 16S/genetics Soil Microbiology
Chemicals
Carbamates DNA, Bacterial DNA, Ribosomal Insecticides RNA, Ribosomal, 16S N-methylcarbamate Carbaryl
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayatsu M
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. ahmhaya@agr.shizuoka.ac.jp
Hirano M
Nagata T
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.65.3.1015-1019.1999
Email
ahmhaya@agr.shizuoka.ac.jp
Published
1999-03-00
Pages
1015-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC91138
Subset
IM
Databases
GENBANK
AB017354
External Links
PubMed source DOI: 10.1128/AEM.65.3.1015-1019.1999 PMC Full Text View journal details
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