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PMID: 10048482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 5 ·No. 6 ·1998-12-31 ·Pages 335-40

Morio T, Urushihara H, Saito T, Ugawa Y, Mizuno H, Yoshida M, Yoshino R, Mitra BN, Pi M, Sato T, Takemoto K, Yasukawa H, Williams J, Maeda M, Takeuchi I, Ochiai H, Tanaka Y

Abstract

In an effort to identify and characterize genes expressed during multicellular development ill Dictyostelium, we have undertaken a cDNA sequencing project. Using size-fractionated subsets of cDNA from the first finger stage, two sets of gridded libraries were constructed for cDNA sequencing. One, library S, consisting of 9984 clones, carries relatively short inserts, and the other, library L, which consists of 8448 clones, has longer inserts. We sequenced all the selected clones in library S from their 3'-ends, and this generated 3093 non-redundant, expressed sequence tags (ESTs). Among them, 246 ESTs hit known Dictyostelium genes and 910 showed significant similarity to genes of Dictyostelium and other organisms. For library L, 1132 clones were randomly sequenced and 471 non-redundant ESTs were obtained. In combination, the ESTs from the two libraries represent approximately 40% of genes expressed in late development, assuming that the non-redundant ESTs correspond to independent genes. They will provide a useful resource for investigating the genetic networks that regulate multicellular development of this organism.

MeSH Terms

Animals Dictyostelium/genetics,growth & development Expressed Sequence Tags Gene Expression Regulation, Developmental Gene Library Molecular Sequence Data Sequence Analysis, DNA Statistics as Topic

Authors & Affiliations

17 authors, click to expand affiliations / ORCID

Morio T

Institute of Biological Sciences, Univ., Tsukuba, Ibaraki, Japan.

Urushihara H

Saito T

Ugawa Y

Mizuno H

Yoshida M

Yoshino R

Mitra B N

Pi M

Sato T

Takemoto K

Yasukawa H

Williams J

Maeda M

Takeuchi I

Ochiai H

Tanaka Y

Article Info

Journal

DNA research : an international journal for rapid publication of reports on genes and genomes

Abbr.

DNA Res

ISSN

1340-2838

DOI

10.1093/dnares/5.6.335

Published

1998-12-31

Pages

335-40

Language

English

Region

England

NLM ID

9423827

Subset

Databases

GENBANK

AU033318, AU033319, AU033320, AU033321, AU033322, AU033323, AU033324, AU033325, AU033326, AU033327, AU033328, AU033329, AU033330, AU033331, AU033332, AU033333, AU033334, AU033335, AU033336, AU033337, AU033338, AU033339, AU033340, AU033341, AU033342, AU033343, AU033344, AU033345, AU033346, AU033347

External Links

PubMed source DOI: 10.1093/dnares/5.6.335 View journal details

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The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development.

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