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题目:: Chromatin immunoprecipitation of mouse polycomb group (PcG) proteins in embryonic stem cells
ID:
状态:: 发布时间May 9, 2006 , 更新时间 June 4, 2014 , 提交时间 May 9, 2006,
物种:: Mus musculus
摘要:: Polycomb group (PcG) proteins are highly conserved from flies to mammals and many of these factors have essential roles in early embryonic development. PcG proteins comprise two multimeric complexes, the Polycomb Repressive complexes 1 and 2 (PRC1 and 2), which have been shown to repress transcription through epigenetic modification of chromatin structure. To gain insight into the role of Polycomb in early development, we have identified PRC1 and PRC2 target genes in mouse embryonic stem (ES) cells using genome-scale location analysis. We found that PRC2 occupies many genes that encode key regulators of development including those encoding transcription factors and components of signaling pathways. These genes are repressed and contain nucleosomes methylated at lysine 27 on histone H3. The majority of PRC2 bound and methylated target genes are co-occupied by PRC1 indicating that these complexes function at a similar set of genes in ES cells. Lack of PRC1 or PRC2 subunits in ES cells results in derepression of target genes and loss of pluripotency. These results provide insight into how PcG proteins contribute to the maintenance of stem cell identity.
实验种类:: ChIP-chip by array
样本量:: 1
实验设计:
: 无设计数据
数据号:: E-WMIT-12
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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