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题目:: RNAi profiling by array of human HEp-2 cells with siRNA-mediated depletion of RNase MRP protein components
ID:
状态:: 发布时间April 1, 2011 , 更新时间 May 2, 2014 , 提交时间 April 22, 2010,
物种:: Homo sapiens
摘要:: To identify mRNA substrates for the human RNase MRP complex, we examined the effects of siRNA-mediated depletion of RNase MRP (and RNase P) on the transcriptome of HEp-2 cells. The expression of two RNase MRP protein components, hPop1 and Rpp40, was knocked-down and after 48 hours mRNA was isolated from these cells as well as from cells transfected with an siRNA targeting the GFP mRNA (siEGFP), which was used as a control. The expression levels of mRNAs were analyzed on a genome-wide scale using 21K microarrays.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
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数据号:: E-TABM-981
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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