Transcription profiling of juvenile Daphnia manga following exposure to various doses of Lufenuron, a benzoylphenylurea growth inhibitor (using Message Amp II)
Juvenile (<24hr old) Daphnia magna populations (n=25) were exposed (24hr) to 0.00ug, 0.15ug, 0.33ug, 0.65ug lufenuron Benzoylphenylurea growth inhibitor. Transcriptomic responses were screened using a D.magna cDNA microarray and the regulation of gene expression via log fold-change in transcript abundance, assessed. Genes involved in the synthesis of chitin were expected to respond to lufenuron, given the mode of action. Extracted total RNA was amplified (400ng/sample) prior to hybridisation with the expectation that this would increase the abundance of significantly responding genes.