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题目:: Chromatin immunopreciptiation of human lymphoblastoid untreated and treated with aphidicolin (APH), trichostatin A (TSA), or APH plus TSA cells to map the origins of DNA replication at common fragile sites
ID:
状态:: 发布时间Feb. 27, 2010 , 更新时间 May 2, 2014 , 提交时间 Oct. 4, 2007,
物种:: Homo sapiens
摘要:: Map the origins of DNA replication in human cells at 7 common fragile sites and their flanking non-fragile sequences as well as a 200kb containing the rare fragile site FRAXA, and a 1,075kb non-fragile region on chr22 . The origins were mapped in untreated cells, as well as, in cells treated with aphidicolin (APH), trichostatin A (TSA), or APH plus TSA. The origin mapping experiment is based on the fact that during S phase, short newly replicated DNA fragments (300bp-1kb) are generated only at the origins of replication. By combining the nascent strand assay with a tiled microarray platform, we have previoulsy developed a rapid, non-PCR based, high-throughput approach to map active origins in asynchronous human cells (Lucas et al., 2007, AC 17668008).
实验种类:: ChIP-chip by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-TABM-348
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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