Expression profiling of Drosophila twist homozygous mutant embryos in a timecourse of embryogenesis. Four one-hour timepoints were assayed (4-8 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to INDAC oligonucleotide arrays.