Transcription profiling of D.melanogaster homozygous Toll10B gain of function mutants vs. wild type embryos at developmental stages 5-7, 8-10 and 10-12 hours
Expression profiling of Drosophila Toll10B homozygous mutant embryos (carrying a dominant active form of the Toll transmembrane receptor) in a timecourse of embryogenesis. Three one-hour timepoints were assayed (3-6 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to INDAC oligonucleotide arrays.