| GeneLibs

题目:: Transcription profiling of background variability of HFFs
ID:
状态:: 发布时间Nov. 7, 2005 , 更新时间 June 4, 2014 , 提交时间 Nov. 7, 2005,
物种:: Homo sapiens
摘要:: Background variability was determined by hybridizing Cy3- and Cy5-cDNA from the same source (mock infected HFFs) to arrays (HE series). Polyadenylated RNA from 107 confluent HFs was purified by using Oligotex (QIAGEN, Valencia, Calif.) from Trizol (Invitrogen)-extracted total RNA. This RNA was prepared, reverse transcribed, labeled with Cy3-dUTP or Cy5-dUTP (Amersham, Little Chalfont, Buckinghamshire, United Kingdom) by random primed synthesis with DNA pol I Klenow (Amersham Life Science, Inc., Cleveland, Ohio), and hybridized to spotted, human cDNA microarrays as previously described. Sequence-verified human cDNA microarrays (HE and HG series, 31,000 spots; HD51 series, 17,000 spots) were produced at Stanford. Images were collected by using a GenePix 4000B microarray scanner, manually flagged to eliminate poor spots and analyzed by GenePix Pro 2.0 (Axon, Union City, Calif.).
实验种类:: transcription profiling by array
样本量:: 0
实验设计:
: 无设计数据
数据号:: E-SMDB-2961
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息