实验库 数据相关信息
- 题目:
- Transcription profiling of germ-free transgenic mice colonized with genotyped Helicobacter pylori strains from a case-control study of gastric cancer
- ID:
- 状态:
- 发布时间Sept. 2, 2005 , 更新时间 March 27, 2012 , 提交时间 Sept. 2, 2005,
- 物种:
- Helicobacter pylori
- 摘要:
- Helicobacter pylori infects the stomachs of half of all humans. It has a relatively benign relationship with most hosts, but produces severe pathology, including gastric cancer, in others. Identifying the relative contributions of host, microbial and environmental factors to the outcome of infection has been challenging. Here, we describe one approach for identifying microbial genes that affect the magnitude of host responses to infection. Single colony purified H. pylori isolates were obtained from 25 cases and 71 controls in a Swedish case-control study of gastric cancer. Strains were first phenotyped based on their ability to produce adhesins that recognize two classes of human gastric epithelial receptors. Thirteen binding strains and two non-binding controls were then subjected to whole genome genotyping using H. pylori DNA microarrays. A cohort of 'variable' genes was identified based on a microarray-determined call of 'absent' in at least one member of the strain panel. Each strain was subsequently introduced into two types of germ-free transgenic mice, each programmed to express a different host factor postulated to pose increased risk for development of severe pathology. Expression of biomarkers of host defense was quantitated 4 weeks after inoculation and magnitude of the response correlated with bacterial genotype. The proportion of genes encoding HsdS homologs (specificity subunit of hetero-oligomeric type I restriction-modification systems) was significantly higher in the pool of 18 variable genes whose presence directly correlated with a robust host response than their proportion in the remaining 352 members of the variable gene pool. This suggests that the functions of these HsdS homologs may include control of expression of microbial determinants that affect the extent of gastric responses to this potentially virulent pathogen.
- 实验种类:
- transcription profiling by array
- 样本量:
- 0
- 实验设计:
- 无设计数据
- 数据号:
- E-SMDB-1581
- 数据状态:
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