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题目:: Transcription profiling of human testis, primary myeloma cell and normal tissue samples to identify novel cancer-testis genes
ID:
状态:: 发布时间Jan. 20, 2009 , 更新时间 May 2, 2014
物种:: Homo sapiens
摘要:: Cancer-testis (CT) antigens are attractive targets for immunotherapeutic strategies since they are aberrantly expressed in malignant cells and not, or in limited number, in somatic tissues, except germ cells. To identify novel CT genes in multiple myeloma, we used Affymetrix HG-U133 gene expression profiles of 5 testis, 64 primary myeloma cell (MMC) and 24 normal tissue (NT) samples. A 5-filter method was developed to keep known CT genes while deleting non-CT-restricted genes. Starting from 44928 probe sets, including probe sets for 18 known CT genes, we have obtained 82 genes expressed in MMC and testis and not detected in more than 6 NT. This list includes 14 of the 18 known CT genes and 68 novel putative CT genes. Real-time RT-PCR was performed for 34 genes in 12 NT, 3 testis and 5 MMC samples and has validated the CT status of 23/34 genes (67%). We found one novel “testis-restricted” gene (expression in testis and tumor only) – TEX14 –, 8 “tissue-restricted” (mRNA detected in 1 or 2 non-gametogenic tissues), and 7 “differentially expressed” (mRNA detected in three to six non-gametogenic tissues) CT genes. Further studies are warranted to determine the immunogenicity of these novel CT antigen candidates.
实验种类:: transcription profiling by array
样本量:: 85
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数据号:: E-MTAB-81
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