Single-cell RNA sequencing reveals a dynamic stromal niche within the evolving tumour microenvironment

·发布 Jan. 4, 2019 ·更新 Jan. 3, 2019

6614

样本数

6614

实验数

实验描述

We employed single-cell RNA sequencing to understand stromal changes in murine melanomas and draining lymph nodes at single cell resolution at different points of tumour development.

样本属性

age

6 to 12 week

developmental stage

adult

genotype

C57BL/6-Tg(CAG- EGFP)131Osb/LeySopJ (TG-/-), C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ, wild type genotype

growth condition

not transfected, transplanted with B16.F10 melanoma cells grown until neoplasm formation

individual

1104, 1107, 1108, 1110, 1112, 1113, 1115, 1117, 1118, 1120, 1121, 1122, 1124, 1125, 1126, 1127, 1129, 1131, 1134, 1135, 1137, 1138, 1139, 1197, 1200, 1202, 1209, 1235, 1239, 1242, 1265/1274, 1269/1274

organism

Mus musculus

organism part

lymph node, skin

phenotype

CD45+ B220+, CD45+ CD3e+ CD4+, CD45+ CD3e+ CD8a+, CD45+ CD3e- B220- CD11b+, CD45+ CD3e- B220- CD11b+ CD11c+, CD45+ CD3e- B220- CD11c+, CD45+ IL7Ra+ ILCs, CD45- CD31+, CD45- CD31-, CD45- GFP+ CD31+, CD45- GFP+ CD31-

sampling site

neoplasm, normal tissue, normal tissue adjacent to neoplasm

sex

female

strain

C57BL/6

实验信息

登记号

E-MTAB-7427

GEO 编号

ERP112843

实验类型

RNA-seq of coding RNA from single cells

物种

Mus musculus

发布日期

Jan. 4, 2019

更新日期

Jan. 3, 2019

提交者

Mirjana Efremova

外部链接

ArrayExpress 原始页 NCBI GEO：ERP112843

分析服务

Single-cell RNA sequencing reveals a dynamic stromal niche within the evolving tumour microenvironment

联系地址

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