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E-MTAB-7242 ERP111085 ChIP-seq Salmonella enterica subsp. enterica serovar Typhimurium

Nus factors prevent premature transcription termination of bacterial CRISPR arrays

提交 Jan. 31, 2018 ·发布 Oct. 13, 2018 ·更新 Sept. 28, 2018

样本数

实验数

实验描述

We used ChIP-seq to map binding of the CRISPR surveillance complex, Cascade, in a Salmonella enterica serovar Typhimurium strain lacking the gene encoding the endonuclease Cas3. We performed ChIP-seq in strains with wild-type and mutant sequences upstream of the two CRISPR arrays, and in strains with wild-type and mutant nusE genes to determine the impact of Nus factor antitermination on CRISPR array function.

样本属性

genotype

nusE N3H mutant ∆cas3::highP, CRII::highP, F3-casD, nusE N3H mutant ∆cas3::highP, CRII::highP, F3-casD + empty pBAD24, nusE N3H mutant ∆cas3::highP, CRII::highP, F3-casD + pBAD24-nusE, ∆cas3::highP, CRI boxA mutant, CRII::highP, F3-casD, ∆cas3::highP, CRII boxA mutant, CRII::highP, F3-casD, ∆cas3::highP, CRII::highP, F3-casD, ∆cas3::highP, ∆CRI, CRII boxA mutant, CRII::highP, F3-casD, ∆cas3::highP, ∆CRI, CRII::highP, F3-casD

immunoprecipitate

anti-FLAG [m2]

organism

Salmonella enterica subsp. enterica serovar Typhimurium

strain

14028s

实验信息

登记号

E-MTAB-7242

GEO 编号

ERP111085

实验类型

ChIP-seq

物种

Salmonella enterica subsp. enterica serovar Typhimurium

提交日期

Jan. 31, 2018

发布日期

Oct. 13, 2018

更新日期

Sept. 28, 2018

提交者

Anne M Stringer

外部链接

ArrayExpress 原始页 NCBI GEO：ERP111085

分析服务

Nus factors prevent premature transcription termination of bacterial CRISPR arrays

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