实验描述
We performed a large-scale genome-wide characterisation of indels generated following editing with CRISPR/Cas9. We used pools of sgRNAs and performed targeted capture and sequencing of the edited regions in HepG2 cells.
样本属性
cell type
epithelial cell
disease
hepatocellular carcinoma
genotype
CRISPR/Cas9 mediated knockout with sgRNA pool 100-1, CRISPR/Cas9 mediated knockout with sgRNA pool 100-2, CRISPR/Cas9 mediated knockout with sgRNA pool 100-3, CRISPR/Cas9 mediated knockout with sgRNA pool 450-5, CRISPR/Cas9 mediated knockout with sgRNA pool 450-6, CRISPR/Cas9 mediated knockout with sgRNA pool 450-7, wild type genotype