Transcription profiling by array of mouse neural stem cells following CRISPR/Cas9 knockdown of Disp3

DISP3 (PTCHD2), a sterol-sensing domain-containing protein, is highly expressed in neural tissue but its role in neural differentiation is unknown. To determine the effect of DISP3 on neural stem cells phenotype of NS-5 cells, we impaired Disp3 gene using CRISPR/Cas9 technology. Modification of Disp3 expression levels affects NS-5 capacity to differentiate. In differentiated Disp3 sgRNAs cell cultures we observed higher number of highly differentiated astrocytes and oligodendrocytes and neurons with not only increased βIII-tubulin mRNA expression but also with more developed neurites.