ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment
ID:
状态:
发布时间Dec. 1, 2010
, 更新时间 June 3, 2014
物种:
Homo sapiens
摘要:
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. (Note: Raw data files not provided by submitter)