Transcription profiling of mouse HA specific T regulatory cells cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific T effector cells
ID:
状态:
发布时间Sept. 8, 2009
, 更新时间 Jan. 25, 2012
物种:
Mus musculus
摘要:
Expanded HA-specific Thy-1.1 Tregs were cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific Teffs. After 5 days, Tregs were sorted by flow cytometry, based on the expression of the Thy-1.1 congeneic marker. Two biological duplicates were hybridized to the Sentrix BeadChips Array mouse WG-6 v2 (Illumina, USA). Data extraction and normalization was performed using Quantile normalization in the BeadStudio software.