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题目:: Transcription profiling of E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells on a novel microarray design to validate the system
ID:
状态:: 发布时间Jan. 1, 2005 , 更新时间 May 3, 2014 , 提交时间 Oct. 31, 2005,
物种:: Mus musculus
摘要:: E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to detect transcripts from all known genes, as well as about 5,000 potential genes, as identified by the NIA Mouse Gene Index 2.0. Seven external RNA controls were derived from intronic and intergenic yeast genome sequences, and 60-mer probes were spotted 10 time each on the slides. These spike-in RNA controls were diluted to cover 7 orders of magnitude in initial concentration, and used to define a function relating signal intensity to transcript abundance.
实验种类:: transcription profiling by array
样本量:: 13
实验设计:
: 无设计数据
数据号:: E-MNIA-66
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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