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题目:: Transcription profiling of mouse embryonic stem cells with inducible Oct-3/4 or Tcl1 expression or Tcl1 knock out using siRNA
ID:
状态:: 发布时间Nov. 30, 2006 , 更新时间 May 3, 2014 , 提交时间 Oct. 3, 2006,
物种:: Mus musculus
摘要:: POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation assays, this analysis identifies not only primary downstream targets of Oct3/4, but also secondary or tertiary targets. Furthermore, the analysis also reveals that downstream target genes are regulated either positively or negatively by Oct3/4. Identification of a group of genes that show both activation and repression depending on Oct3/4 expression levels provides a possible mechanism for the requirement of appropriate Oct3/4 expression to maintain undifferentiated ES cells. As a proof-of-principle study, one of the downstream genes, Tcl1, has been analyzed in detail. We show that Oct3/4 binds to the promoter region of Tcl1 and activates its transcription. We also show that Tcl1 is involved in the regulation of proliferation, but not differentiation, in ES cells. These findings suggest that the global expression profiling of gene-manipulated ES cells can help to delineate the structure and dynamics of gene regulatory networks.
实验种类:: transcription profiling by array
样本量:: 39
实验设计:
: 无设计数据
数据号:: E-MNIA-61
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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