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题目:: RNAi knock down of daf-16 in C. elegans over 0-192 hours
ID:
状态:: 发布时间July 17, 2003 , 更新时间 May 2, 2014
物种:: Caenorhabditis elegans
摘要:: Comparison of sterile worms grown on Control Vector, daf-2 RNAi, and daf-2 + daf-16 RNAi. Synchronized fer-15(b26); fem-1(hc17) animals were grown on RNAi bacteria at 25ºC, induced with IPTG on Day 1 of adulthood, and collected from 0-48 hours (10 time points) and 0-196 hours (10 time points); RNAi bacteria was supplemented as necessary for later time points. Worms were floated off lawns with M9 buffer, centrifuged, and washed again in M9. The pelleted worms were dissolved in Trizol (Gibco) and frozen in liquid nitrogen.Time courses were compared with mixed references (collected from 1/2 of each of the time course samples)
实验种类:: RNAi profiling by array
样本量:: 30
实验设计:
: 无设计数据
数据号:: E-MEXP-9
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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